# US 11,298,395 B2 · Isolates from spent turmeric for the management of rheumatoid arthritis

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- Number: US 11,298,395 B2
- Document ID: US11298395B2
- Status: Granted
- Kind: B2
- Application number: 16/632,953
- Filed: 2018-07-17
- Published: 2020-05-28
- Granted: 2022-04-12
- Earliest claimed: 2017-07-24
- Assignees: Sami Labs Limited
- Applicants: Sami Labs Limited
- Inventors: Muhammed Majeed; Kalyanam Nagabhushanam; Beena Bhat; Natarajan Sankaran
- Web page: https://patentgrep.com/patent/US11298395B2

## CPC

- A61K 36/9066: Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
- A61K 31/198: Alpha-amino acids, e.g. alanine or edetic acid [EDTA] (betaine A61K31/205; proline A61K31/401; tryptophan A61K31/405; histidine A61K31/4172; peptides not degraded to individual amino acids A61K38/00)
- A61K 31/715: Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K 31/718: Starch or degraded starch, e.g. amylose, amylopectin
- A61P 19/02: for joint disorders, e.g. arthritis, arthrosis
- A61P 29/00: Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
- C07K 14/415: from plants
- C08B 30/20: Amylose or amylopectin (chemical derivatives thereof C08B33/00, C08B35/00)
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- A61K 2236/00: Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K 2236/331: using water, e.g. cold water, infusion, tea, steam distillation or decoction
- A61K 2236/39: Complex extraction schemes, e.g. fractionation or repeated extraction steps
- A61K 2236/51: Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
- A61K 2236/53: Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C08B: POLYSACCHARIDES; DERIVATIVES THEREOF (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages C07H; fermentation or enzyme-using processes C12P19/00; sugar industry C13; production of cellulose D21)

## Abstract

The present invention relates to a process for the isolation of bioactive components from spent turmeric (Curcuma longa) and compositions comprising said bioactive components. Further, the present invention also elucidates the potent anti-inflammatory activity of said bioactive compositions and therapeutic applications thereof in rheumatoid arthritis.

## Claims

### Claim 1

A method of treating rheumatoid arthritis in mammals, said method comprising step of administering to said mammals effective amounts of spent turmeric protein or spent turmeric starch, said protein and starch obtained by process comprising steps of
a) Extracting spent turmeric with water wherein ratio of spent turmeric and water is from about 1:10 to about 1:50, at a temperature 65° C. for 6 hours with occasional stirring to get water extract of spent turmeric and a residual material, wherein the residual material is spent turmeric material-2;
b) Centrifuging the water extract of step (a) to trap sand or dust particles to obtain a clear filtrate;
c) Concentrating the clear filtrate of step (b) at 60° C. with high vacuum to obtain a concentrate and further cooling said concentrate;
d) Adding acetone, to the concentrate of step (c) wherein ratio of acetone and concentrate, is 1:2, followed by occasional stirring, incubation at room temperature for 4 hours to form acetone precipitated protein and allowing the precipitated protein to completely settle down with a supernatant above;
e) Decanting the supernatant of step (d) without disturbing the precipitated protein followed by filtration, washing the precipitated protein with acetone to remove moisture and filtering again;
f) Drying protein precipitate of step (e) immediately at 60° C. with high vacuum to obtain fine protein (glycoprotein) powder as an extract, wherein the extract reduces inflammation and symptoms of rheumatoid arthritis in said mammals including pain, swelling, oedema and reduction in serum markers of IL-6 and TNF-α;
g) Subjecting the residual spent turmeric material-2 of step (a) devoid of protein of step (f) to washing with soaking volume of Acetone and Methanol for 4 hours each at 46° C. and 30° C. respectively to remove traces of curcuminoids and oxalic acid content to form washed spent turmeric material-2; and
h) Drying washed spent turmeric material-2 of step (g) for 24 hours to remove residue solvents followed by powdering and sieving to get turmeric starch (polysaccharide) as an extract wherein polysaccharide is attached to hydrophobic protein, wherein the extract reduces inflammation and symptoms of rheumatoid arthritis in said mammals including pain, swelling, oedema and reduction in serum markers of IL-6 and TNF-α.