Recombinant Bacillus subtilis with improved 2′-fucosyllactose production, and construction method thereof

A recombinant Bacillus subtilis with improved 2′-fucosyllactose production, wherein the recombinant Bacillus subtilis is obtained by over-expressing a fusion protein of L-fucokinase/guanosine 5′-diphosphate-L-fucose pyrophosphorylase and a fucosyltransferase in Bacillus subtilis BSGL,

• wherein the L-fucokinase/guanosine 5′-diphosphate-L-fucose pyrophosphorylase is conjugated to the fucosyltransferase (FutC) by a linker;
• wherein the L-fucokinase/guanosine 5′-diphosphate-L-fucose pyrophosphorylase is expressed by the gene having the nucleotide sequence of GenBank:
• AY849806.1 on NCBI as of May 25, 2020;
• and the fucosyltransferase is expressed by the gene having the nucleotide sequence of GenBank:
• KY499613 on NCBI as of May 25, 2020;
• and
• wherein the linker has an amino acid sequence selected from the group consisting of SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6.