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US 11,293,009 B2Granted
Remnant tumor infiltrating lymphocytes and methods of preparing and using the same
AssigneeIovance Biotherapeutics, Inc.
InventorsMichelle R. Simpson-Abelson, Christopher Mosychuk, Michael T. Lotze
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Abstract
In some embodiments, methods of delivering a therapeutically effective amount of an expanded number of tumor infiltrating lymphocytes obtained from tumor remnants to a patient in need thereof, for the treatment of a cancer, are disclosed.
Claims22
A method of treating a cancer in a patient in need of such treatment, the method comprising:
- (a) obtaining tumor tissue from the patient, wherein the tumor tissue comprises tumor infiltrating lymphocytes (TILS);
- (b) fragmenting the tumor tissue to provide fragmented tumor tissue;
- (c) treating the fragmented tumor tissue in a gas permeable container with a first cell culture medium and interleukin 2 (IL-2) to provide emergent TILS (eTILs) and tumor remnants comprising remnant TILs (rTILs);
- (d) removing at least a plurality of the eTILs;
- (e) enzymatically digesting the tumor remnants into tumor remnant cells using a digest mixture, wherein the digest mixture comprises a deoxyribonuclease, a collagenase, a hyaluronidase, or a combination thereof;
- and
- (f) expanding the tumor remnant cells with a second cell culture medium comprising cell culture media, irradiated feeder cells, OKT-3 antibody, and IL-2 in a gas permeable container to provide an expanded number of the rTILs for administration to the patient;
- and
- (g) administering a therapeutically effective amount of the rTILs to the patient.
6 more elements
Dependencies are inferred from the claim text.