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US 11,286,474 B2Granted
Enzyme products
AssigneeC-LEcta GmbH
InventorsStefan Schoenert, Mathias Salomo, Thomas Schultchen, Andreas Vogel, Sabrina Koepke, Sebastian Bartsch, Birgit Brucher, Claudia Feller
Life of this patent
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Abstract
The invention relates to a process for the manufacturing and purification of recombinant enzyme products, in particular of food enzyme products and the use thereof. The invention particularly relates to a process for the processing of enzyme products from a microbial fermentation broth by methods of separation, enzymatic treatment and filtration procedures.
Claims21
A process for preparing a recombinant enzyme formulation comprising:
- (i) providing a composition I comprising a recombinant enzyme, nucleic acids, and optionally cell debris;
- (ii) adding to the composition I a nuclease wherein the nuclease breaks down the nucleic acids to result in a composition II comprising the recombinant enzyme, broken down nucleic acids, and optionally the cell debris;
- (iii) subsequently, adding to the composition II a precipitation agent for the broken down nucleic acids wherein the broken down nucleic acids are complexed resulting in a composition III comprising the recombinant enzyme, complexed broken down nucleic acids, and optionally the cell debris;
- (iv) optionally, purifying the composition III by solid/liquid separation resulting in a separated solid phase comprising the complexed broken down nucleic acids and optionally the cell debris and a liquid composition IV comprising the recombinant enzyme;
- and
- (v) subsequently, purifying the composition III or the composition IV by microfiltration resulting in a composition V, the recombinant enzyme formulation, comprising the recombinant enzyme.
3 more elements
Dependencies are inferred from the claim text.