System and method for production of fatty acid ethanolamides
AssigneeUnited Arab Emirates University
InventorsMohammed Kalim Akhtar, Dhanya Vijay
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Abstract
A system and method for production of fatty acid ethanolamides (FAEs) are provided. The system and method for production of fatty acid ethanolamides includes microbes engineered to express the necessary enzymes to complete a FAE biosynthetic pathway, resulting in the conversion of glucose to one or more desired FAEs. The microbes may be engineered to express at least two of thioesterase, fatty acyl CoA synthase, NAPE-synthase, and NAPE phospholipase-D. The FAE-producing microbes can be administered to a subject in need thereof as part of a probiotic regimen.
Claims13
A system for production of fatty acid ethanolamides, comprising a microbe engineered to express thioesterase, fatty acyl CoA synthase, N-acylated phosphatidylethanolamine synthase, and NAPE phospholipase-D.
A probiotic composition comprising a microbe engineered to express a fatty acid ethanolamide (FAE)-producing pathway comprising thioesterase, fatty acyl CoA synthase, N-acylated phosphatidylethanolamine synthase, and NAPE phospholipase-D and a pharmaceutically acceptable carrier.
A method of designing a system for production of fatty acid ethanolamides, comprising:
- selecting a microbe that naturally produces fatty acid ethanolamide precursors;
- and genetically engineering the microbe to express a FAE-producing pathway comprising thioesterase, fatty acyl CoA synthase, N-acylated phosphatidylethanolamine synthase, and NAPE phospholipase-D.
Dependencies are inferred from the claim text.