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US 11,286,464 B2Granted
Viral vaccines and methods of forming the same
AssigneeNDSU Research Foundation
InventorSheela Ramamoorthy
Life of this patent
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Abstract
Provided herein is a method for producing an inactivated virus including a) heating the virus to a temperature sufficient to disrupt the virus membrane; b) exposing the virus of step (a) to an RNA degrading enzyme; and c) cooling the virus to a temperature sufficient to reestablish the integrity of the virus membrane. Also provided herein is a vaccine produced using the instant method.
Claims18
A method for producing an inactuated virus comprising:
- a) forming a denatured viral capsid through heating of the virus to a temperature of between 40° C. and about 44° C. for between 5 and 30 minutes;
- b) exposing viral genetic material within the denatured viral capsid of step (a) to a ribonucleic acid (RNA) degrading enzyme;
- and
- c) following steps (a) and (b), reestablishing the integrity of the denatured viral capsid through cooling of the virus;
- wherein exposing the viral genetic material to the RNA degrading enzyme in step (b) at least partially destroys the viral genetic material within the denatured viral capsid;
- and
- wherein steps (a)-(c) are performed without damaging the immunogenic capsid antigens of the virus.
4 more elements
A method for producing an inactuated virus comprising:
- a) heating the virus to a temperature of about 42° C. for at least 10 minutes to denature the viral capsid;
- b) exposing viral genetic material within the virus of step (a) to a ribonucleic acid (RNA) degrading enzyme;
- and
- c) cooling the virus to a temperature of 4° C. or less for at least 3 hours to reestablish the integrity of the denatured viral capsid;
- wherein the exposing in step (b) at least partially destroys the viral genetic material.
2 more elements
Dependencies are inferred from the claim text.