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US 11,286,457 B1Granted
Methods of improved yeast health and fermentation with urease
AssigneePOET Research, Inc.
InventorsStephen M. Lewis, Camille K. Crouch, Benjamin P. Gacke
Life of this patent
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Abstract
Methods, compositions, and systems for propagation and fermentation, particularly large scale operations for production of ethanol and dried distiller's grain are provided. Addition of urease and urea to propagation and/or fermentation improves yeast health, fermentation efficiency, and quality and quantity of DDG. Urease can be in reagent form or can be endogenous to the natural feedstock.
Claims20
A composition comprising:
- (a) a feedstock comprising (i) milled corn grain and (ii) enzyme active whole soybean or enzyme active de-hulled soybean, (b) urease, wherein the urease is endogenous to the soybean, (c) urea, (d) a microorganism, and (e) water, further comprising one or more of a cellulase, lipase, protease, and phytase.
A composition comprising:
- (a) a feedstock comprising (i) milled corn grain and (ii) enzyme active whole soybean or enzyme active de-hulled soybean comprising endogenous urease, (b) urea, (c) a microorganism, and (d) water;
- wherein the enzyme active whole soybean or enzyme active de-hulled soybean is obtained from soybean not exposed to temperatures greater than 71° C.
A method of improving fermentation efficiency comprising:
- (I) combining (a) a feedstock comprising (i) milled corn grain and (ii) enzyme active whole soybean or enzyme active de-hulled soybean, (b) urease, wherein the urease is endogenous to the soybean, (c) urea, (d) a microorganism, (e) one or more of a cellulase, lipase, protease, and phytase, and (f) water in a fermenter;
- and
- (II) fermenting the contents of the fermenter;
- wherein the fermentation efficiency is improved relative to the fermentation efficiency achieved in the absence of urea and urease.
1 more element
A method of improving fermentation efficiency comprising:
- (I) combining (a) a feedstock comprising (i) milled corn grain and (ii) enzyme active whole soybean or enzyme active de-hulled soybean comprising endogenous urease, (b) urea, (c) a microorganism, and (d) water in a fermenter;
- and
- (II) fermenting the contents of the fermenter;
- wherein the enzyme active whole soybean or enzyme active de-hulled soybean is not exposed to temperatures greater than 71° C.;
- and
- wherein the fermentation efficiency is improved relative to the fermentation efficiency achieved in the absence of urea and urease.
3 more elements
Dependencies are inferred from the claim text.