Back to results
US 11,285,193 B2Granted
Methods and compositions for RNA-guided treatment of HIV infection
AssigneeTemple University of the Commonwealth System of Higher Education
InventorsKamel Khalili, Wenhui Hu
Life of this patent
Estimating the term…
Abstract
A method of treating a subject at risk for having a virus infection, by administering to the subject a prophylactically effective amount of a composition comprising a vector encoding a CRISPR-associated endonuclease and at least two guide RNAs, wherein the guide RNAs are complementary to two target sequences spanning from the 5′- to 3′-LTRs of the sequence in the virus, and preventing a retroviral infection.
Claims8
A method of excising a human immunodeficiency virus (HIV) sequence from a genome of a cell, the method comprising contacting the cell with:
- (a) a Clustered Regularly Interspace Short Palindromic Repeat (CRISPR)-associated Cas9 endonuclease or a nucleic acid sequence encoding the CRISPR-associated Cas9 endonuclease;
- and
- (b) two or more guide RNAs (gRNAs) or one or more nucleic acid sequences encoding the two or more guide gRNAS, at least two of the two or more guide RNAs targeting a cleavage site, (i) a first guide RNA or a nucleic acid sequence encoding the first gRNA, the first gRNA being complementary to a first target nucleic acid sequence within a 5′ long terminal repeat (LTR) of the HIV sequence;
- and (ii) a second gRNA or a nucleic acid sequence encoding the second gRNA, the second gRNA being complementary to a 3′ long terminal repeat (LTR) of the HIV sequence, at least two of the two or more guide RNAs being different, and
- wherein the method excises the HIV sequence between cleavage sites from the genome of the cell.
2 more elements
Dependencies are inferred from the claim text.