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US 11,282,587 B2Granted
Automated priming and library loading device
AssigneeClear Labs, Inc.
InventorsSasan Amini, Ramin Khaksar, Michael Taylor, Shadi Shokralla, Christopher Haney, Pavan Vaidyanathan, Stephanie Pollard, Adam Allred, Sima Mortazavi, David Tran, Hossein Namazi, Julius Barsi
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Abstract
Provided herein are automated apparatus for the identification of microorganisms in various samples. The disclosure solves existing challenges encountered in identifying and distinguishing various types of microorganisms, including viruses and bacteria in a timely, efficient, and automated manner by sequencing.
Claims28
A method of reusing a flow cell in a sequencing reaction, the method comprising:
- (a) adding a first barcode to a first plurality of nucleic acid sequences from a first sample, thereby providing a first plurality of barcoded nucleic acid sequences;
- (b) performing a first sequencing reaction on said first plurality of barcoded nucleic acid sequences on a sequencing apparatus comprising a flow cell;
- (c) adding a second barcode to a second plurality of nucleic acid sequences from a second sample, thereby providing a second plurality of barcoded nucleic acid sequences;
- and
- (d) performing a second sequencing reaction on said second plurality of barcoded nucleic acid sequences on said sequencing apparatus comprising said flow cell, thereby reusing said flow cell of said sequencing apparatus,
- wherein said first barcode comprises a first plurality of blocks and said second barcodes comprises a second plurality of blocks, and wherein a block within said first or said second plurality of blocks has a maximum Levenshtein distance from another block within said plurality of blocks.
3 more elements
Dependencies are inferred from the claim text.