Anti-VLA-4 related assays
AssigneeBiogen MA Inc.
InventorsTatiana Plavina, Paula S. Hochman, Michaela Lerner
Life of this patent
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Abstract
Methods and apparatus for assaying the level of analytes in a sample, related to VLA-4, are disclosed. A method of decreasing the level of an anti-integrin antibody in a subject is described including a) contacting a biological sample from a subject with a detectable capture agent associated with a substrate, wherein the capture agent can bind an anti-integrin antibody in the sample; b) detecting binding of the capture agent with the level of the anti-integrin antibody; and c) treating the subject with plasma exchange until the level of the anti-integrin antibody in the sample reaches a predetermined level.
Claims21
A method of treating a subject to reduce a level of anti-VLA-4 antibody in the subject, the method comprising treating the subject using blood apheresis to reduce the level of anti-VLA-4 antibody in the subject until the level of anti-VLA-4 antibody is below a predetermined level, wherein
- a. the subject is a subject having undergone treatment with the anti-VLA-4 antibody;
- and
- b. the level of anti-VLA-4 antibody is determined by:
- i. obtaining a biological sample from the subject;
- ii. contacting the sample with a capture agent immobilized on a substrate, wherein the capture agent can bind the anti-VLA-4 antibody in the sample, and wherein the substrate is a portion of a lateral flow immunochromatographic system comprising, in order of flow, a label zone, a sample receiving zone, and a test zone configured such that:
- the label zone is located upstream of the sample receiving zone and contains one or more labeled antibodies, the test zone is downstream of the label zone, upon flow through the lateral flow immunochromatographic system the sample reaches the test zone before labeled antibody(s) from the label zone upon flow through the lateral flow immunochromatographic system, and upon flow of the sample through the system, anti-VLA-4 antibody from the sample will first bind with the capture agent in the test zone to form a complex, the labeled antibody(s) will then reach the test zone, and then the complex will bind with the labeled antibody(s);
- and
- iii. detecting binding of the anti-VLA-4 antibody to the capture agent with the labeled antibody(s) within the lateral flow immunochromatographic system and determining whether the level of the anti-VLA-4 antibody is above or below the predetermined level based on the detecting.
A method of treating a subject to reduce a level of anti-VLA-4 antibody in the subject, the method comprising:
- a. selecting a subject who has been treated with the anti-VLA-4 antibody and has been identified as having a level of anti-VLA-4 antibody above a predetermined level;
- and then
- b. treating the subject using blood apheresis until the level of anti-VLA-4 antibody is below the predetermined level, wherein the level of anti-VLA-4 antibody has been determined by:
- i. obtaining a biological sample from the subject;
- ii. contacting the sample with a capture agent immobilized on a substrate, wherein the capture agent can bind the anti-VLA-4 antibody in the sample, and wherein the substrate is a portion of a lateral flow immunochromatographic system comprising, in order of flow, a label zone, a sample receiving zone, and a test zone configured such that:
- the label zone is located upstream of the sample receiving zone and contains one or more labeled antibodies, the test zone is downstream of the label zone, upon flow through the lateral flow immunochromatographic system the sample reaches the test zone before labeled antibody(s) from the label zone upon flow through the lateral flow immunochromatographic system, and upon flow of the sample through the system, anti-VLA-4 antibody from the sample will first bind with the capture agent in the test zone to form a complex, the labeled antibody(s) will then reach the test zone, and then the complex will bind with the labeled antibody(s);
- and
- iii. detecting binding of the anti-VLA-4 antibody to the capture agent with the labeled antibody(s) within the lateral flow immunochromatographic system and determining whether the level of the anti-VLA-4 antibody is above or below the predetermined level based on the detecting.
A method of detecting a level of an anti-anti-VLA-4 antibody in a sample from a subject, wherein
- a. the subject is a subject having undergone treatment with the anti-VLA-4 antibody;
- and
- b. the level of anti-anti-VLA-4 antibody is determined by:
- i. obtaining a biological sample from the subject;
- ii. contacting the sample with a capture agent immobilized on a substrate, wherein the capture agent can bind the anti-anti-VLA-4 antibody in the sample, and wherein the substrate is a portion of a lateral flow immunochromatographic system comprising, in order of flow, a label zone, a sample receiving zone, and a test zone configured such that:
- the label zone is located upstream of the sample receiving zone and contains one or more labeled antibody(s), the test zone is downstream of the label zone, upon flow through the lateral flow immunochromatographic system the sample reaches the test zone before labeled antibody(s) from the label zone upon flow through the lateral flow immunochromatographic system, and upon flow of the sample through the system, anti-anti-VLA-4 antibody from the sample will first bind with the capture agent in the test zone to form a complex, the labeled antibody(s) will then reach the test zone, and then the complex will bind with the labeled antibody(s)secondary agent(s);
- and
- iii. detecting binding of the anti-anti-VLA-4 antibody to the capture agent with the labeled antibody(s) within the lateral flow immunochromatographic system and determining the level of the anti-anti-VLA-4 antibody based on the detecting.
Dependencies are inferred from the claim text.