Method of producing xylo-oligosaccharide
AssigneeToray Industries, Inc.
InventorsTakuya Kasahara, Chiaki Yamada, Hiroyuki Kurihara, Katsushige Yamada
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Abstract
A method produces xylo-oligosaccharides from a biomass containing xylan and cellulose, which method is convenient and has a high yield of xylo-oligosaccharides because of inhibition of degradation of xylo-oligosaccharides into xylose. In the method of producing xylo-oligosaccharides, a biomass containing xylan and cellulose is hydrolyzed with a cellulase composition having at least activities of xylanase, cellobiohydrolase and β-glucosidase and substantially free of β-xylosidase activity during hydrolysis.
Claims10
A method of producing a cellulase composition, comprising incubating a cellulase mixture not containing a biomass containing xylan and cellulose, having activities of xylanase, cellobiohydrolase, β-glucosidase and β-xylosidase originated from the genus Trichoderma and β-glucosidase activity originated from the genus Aspergillus at a temperature of 35° C. to 60° C. and pH of 5.5 to 8.0 to selectively reduce the activity of β-xylosidase while maintaining the activity of β-glucosidase, xylanase and cellobiohydrolase, to attain
- a β-glucosidase activity in said cellulase composition in terms of an enzyme activity for degrading 4-nitrophenyl-β-D-glucopyranoside of at least 14,000 U/g of proteins in said cellulase composition;
- a β-xylosidase activity m said cellulase composition in terms of an enzyme activity for degrading 4-nitrophenyl-β-D-xylopyranoside of 50 to 300 U/g of proteins in said cellulase composition;
- a xylanase activity in said cellulase composition in terms of an enzyme activity for degrading xylan of at least 18,000 U/g of proteins in said cellulase composition;
- and
- a cellobiohydrolase activity in said cellulase composition in terms of an enzyme
- activity for degrading 4-nitrophenyl-β-D-lactopyranoside of at least 80 U/g of proteins in said cellulase composition.
A method of producing cellulase composition comprising:
- a) incubating a cellulase mixture not containing a biomass containing xylan and cellulose, having the activities of xylanase, cellobiohydrolase, β-glucosidase and β-xylosidase activities originated from genus Trichoderma at a temperature of 35° C. to 60° C. and pH of 5.5 to 8.0 to selectively reduce the activity of both β-glucosidase and β-xylosidase while maintaining the activity of xylanase and cellobiohydrolase;
- and
- b) adding β-glucosidase into the cellulase mixture of a), to attain a β-glucosidase activity in said cellulase composition in terms of an enzyme activity for degrading 4-nitrophenyl-β-D-glucopyranoside of at least 14,000 U/g of proteins in said cellulase composition;
- a β-xylosidase activity in said cellulase composition in terms of an enzyme activity for degrading 4-nitrophenyl-β-D-xylopyranoside of 50 to 300 U/g of proteins in said cellulase composition;
- a xylanase activity in said cellulase composition in terms of an enzyme activity for degrading xylan of at least 18,000 U/g of proteins in said cellulase composition;
- and
- a cellobiohydrolase activity in said cellulase composition in terms of an enzyme activity for degrading 4-nitrophenyl-β-D-lactopyranoside of at least 80 U/g of proteins in said cellulase composition.
Dependencies are inferred from the claim text.