Maize RBCS7A promoter for expressing a ferredoxin-thioredoxin reductase in a plant
AssigneeBenson Hill, Inc.
InventorMatthew Begemann
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Abstract
Compositions and methods for improving plant growth are provided herein. The maize RbcS7A promoter is provided which is a developmentally regulated promoter. Polynucleotides encoding ferredoxin-thioredoxin reductase proteins, polypeptides encompassing ferredoxin-thioredoxin reductase proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
Claims15
A method for increasing crop yield comprising transforming a plant with at least one ferredoxin-thioredoxin reductase protein-encoding sequence, wherein said ferredoxin-thioredoxin reductase protein-encoding sequence encodes a protein comprising an amino acid sequence having at least 90% sequence identity to a selected from the group consisting of SEQ ID NOs:2 and 14-106, wherein said protein has ferredoxin-thioredoxin reductase activity;
- and
- wherein said ferredoxin-thioredoxin reductase protein-encoding sequence is operably linked to a promoter comprising the nucleic acid sequence set forth SEQ ID NO:
- 5.
A plant having stably incorporated into its genome a promoter that drives expression in a plant operably linked to a ferredoxin-thioredoxin reductase protein-encoding sequence, wherein said promoter is heterologous to said ferredoxin-thioredoxin reductase protein-encoding sequence, wherein said ferredoxin-thioredoxin reductase protein-encoding sequence encodes a protein comprising an amino acid sequence having at least 90% sequence identity to a selected from the group consisting of SEQ ID NOs:2 and 14-106, wherein said protein has ferredoxin-thioredoxin reductase activity;
- and
- wherein said promoter comprises the nucleic acid sequence set forth SEQ ID NO:
- 5.
A DNA construct comprising, in operable linkage,
- a. A promoter that is functional in a plant cell, wherein said promoter comprises the nucleic acid sequence set forth SEQ ID NO:
- 5;
- and,
- b. A nucleic acid sequence encoding a ferredoxin-thioredoxin reductase protein, wherein said nucleic acid sequence encoding a ferredoxin-thioredoxin reductase protein encodes a protein comprising an amino acid sequence having at least 90% sequence identity to a selected from the group consisting of SEQ ID NOs:2 and 14-106, wherein said protein has ferredoxin-thioredoxin reductase activity.
Dependencies are inferred from the claim text.