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US 11,278,901 B2Granted
Microfluidic surface-mediated emulsion stability control
Assignee10x Genomics, Inc.
InventorsRajiv Bharadwaj, Tobias Daniel Wheeler, Kevin Ness, Benjamin Hindson
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Abstract
A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.
Claims18
A method of emulsion droplet surface-mediated coalescence control comprising:
- a) providing a microfluidic emulsion droplet generation system comprising:
- i) a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation;
- ii) at least one channel junction in the flow path for emulsion droplet formation;
- and
- iii) a reservoir in the flow path downstream of the channel junction comprising at least one textured surface configured and arranged for inducing surface-mediated coalescence of a plurality of emulsion droplets;
- b) providing a dispersed phase and a continuous phase to the system for emulsion droplet formation;
- c) forming emulsion droplets in the system;
- d) holding the emulsion droplets in the flow path for a time;
- and
- e) directing the emulsion droplets to the textured surface, thereby coalescing the emulsion droplets.
7 more elements
Dependencies are inferred from the claim text.