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US 11,274,351 B2Granted
Methods and compositions for detecting bacterial contamination
AssigneeDch Molecular Diagnostics, Inc.
InventorsJames Jian Quan Wang, Xiangmin Cui, Wayne Jer Hsieh, HiuNam Chan
Life of this patent
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Abstract
Compositions, reactions mixtures, kits, and systems for detecting bacterial contamination are provided, as well as methods of using the same.
Claims19
A composition for detecting bacterial contamination of a platelet sample, comprising:
- a single primer pair that, in an amplification reaction with a 16S ribosomal ribonucleic acid (16S rRNA) polynucleotide, amplifies an amplicon of at least 50 nucleotides in length;
- and
- a single detectable probe that hybridizes to either strand of the amplicon;
- wherein the single primer pair having a first primer and a second primer flanks the amplicon that comprises a conserved sequence to which the single detectable probe hybridizes;
- wherein the first primer and the second primer each hybridizes to a separate conserved region of the 16S rRNA polynucleotide;
- wherein the separate conserved region is identical among at least five bacterial genera;
- and wherein the separate conserved region is (i) selected from the group consisting of from 9 to 28, from 32 to 48, from 888 to 903, from 908 to 937, from 975 to 994, from 957 to 981, from 1093 to 1125, from 1184 to 1206, from 1231 to 1252, from 1378 to 1396, from 1398 to 1422, and from 1496 to 1516, of 16S rRNA of Staphylococcus aureus (GenBank accession Number NC_007622) or (ii) a corresponding region in a genome of any of the at least five bacterial genera,
- wherein hybridization of the single detectable probe to the either strand of the amplicon yields a detectable signal indicative of bacterial contamination of the platelet sample by the any of the at least five bacterial genera.
5 more elements
A composition for detecting bacterial contamination of a platelet sample, comprising:
- a single primer pair that, in an amplification reaction with a 16S ribosomal ribonucleic acid (16S rRNA) polynucleotide, amplifies an amplicon of at least 50 nucleotides in length;
- and
- a single detectable probe that hybridizes to either strand of the amplicon;
- wherein the single primer pair flanks the amplicon that comprises a conserved sequence to which the single detectable probe hybridizes;
- and the conserved sequence is identical among at least five bacterial genera, wherein the single detectable probe comprises at least 10 contiguous nucleotides of a sequence selected from the group consisting of SEQ ID:
- 11, SEQ ID:
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- 127, SEQ ID:
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- 154, SEQ ID:
- 155, and a complement thereof,
- wherein hybridization of the single detectable probe to the either strand of the amplicon yields a detectable signal indicative of bacterial contamination of the platelet sample by any of the at least five bacterial genera.
38 more elements
Dependencies are inferred from the claim text.