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US 11,274,324 B2Granted
Engineering polyketide synthase in cyanobacteria
AssigneeThe Regents of the University of California
InventorsMichael D. Burkart, Jeffrey Mindrebo, James Golden, Arnaud Taton, Julia Roulet, Hugo Gramajo, Ana Arabolaza
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Abstract
Provided herein, inter alia, is a modular-functional technology for the expression of a functional heterologous polyketide synthases (PKS) system in a photosynthetic cyanobacteria.
Claims19
A recombinant cyanobacterium comprising:
- (a) an exogenous gene capable of expressing an exogenous Sfp-type phosphopantetheinyl transferase (PPTase);
- (b) one or more plasmids comprising a plurality of genes expressing a plurality of enzymes that work together to produce a carboxyacyl-CoA compound from a carboxyacyl-CoA precursor, wherein the plurality of genes comprise:
- (i) matB and epimerase;
- (ii) mutA, mutB, and epimerase;
- or
- (iii) accA2, pccB, pccE, and acsA;
- and
- (c) an exogenous gene capable of expressing an enzyme that catalyzes the formation of RNA from DNA in the 5′→3′ direction.
5 more elements
A recombinant cyanobacterium comprising:
- (a) an exogenous gene capable of expressing an exogenous Sfp-type phosphopantetheinyl transferase (PPTase);
- (b) one or more plasmids comprising a plurality of genes expressing a plurality of enzymes that work together to produce a carboxyacyl-CoA compound from a carboxyacyl-CoA precursor, wherein the plurality of genes comprise:
- (i) matB and epimerase;
- or
- (ii) accA2, pccB, pccE, and acsA.
2 more elements
A recombinant cyanobacterium comprising:
- (a) an exogenous gene capable of expressing an exogenous Sfp-type phosphopantetheinyl transferase (PPTase);
- and
- (b) one or more plasmids comprising a plurality of genes expressing a plurality of enzymes that work together to produce a carboxyacyl-CoA compound from a carboxyacyl-CoA precursor, wherein the plurality of genes comprise:
- (i) matB and epimerase;
- (ii) mutA, mutB, and epimerase;
- or
- (iii) accA2, pccB, pccE, and acsA;
- wherein the cyanobacterium is of the genus Synechococcus.
5 more elements
Dependencies are inferred from the claim text.