Biosynthesis of cannabinoids and cannabinoid precursors
AssigneeGinkgo Bioworks, Inc.
InventorsKim Cecelia Anderson, Elena Brevnova, Dylan Alexander Carlin, Brian Carvalho, Nicholas Flores, Katrina Forrest, Massimo Merighi, Gabriel Rodriguez, Emily E. Wrenbeck
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Abstract
Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro.
Claims20
A method for producing a cannabinoid compound or a cannabinoid precursor comprising culturing a host, cell that comprises a heterologous polynucleotide encoding a polyketide synthase (PKS), wherein the PKS comprises an amino acid sequence that has at least 95% sequence identity to SEQ ID NO:
- 7, wherein the cannabinoid compound is a compound of Formulas 8, 9, 10, or 11:
- or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof;
- wherein the cannabinoid precursor is a compound of Formulas 4, 5, or 6:
- or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof;
- wherein R is straight-chain unsubstituted C1-20 alkyl, and
- wherein the host cell is not a mammalian cell.
A method for producing a cannabinoid compound or a cannabinoid precursor comprising culturing a host cell that comprises a heterologous polynucleotide encoding a polyketide synthase (PKS), wherein the PKS comprises SEQ ID NO:
- 7 or a conservatively substituted version thereof, wherein the cannabinoid compound is a compound of Formulas 8, 9, 10, or 11:
- or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof:
- wherein the cannabinoid precursor is a compound of Formulas 4, 5, or 6:
- or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof:
- wherein R is straight-chain unsubstituted C1-20 alkyl, and
- wherein the host cell is not a mammalian cell.
Dependencies are inferred from the claim text.