RNA engineered T cells for the treatment of cancer

An in vitro transcribed RNA or synthetic RNA, comprising a nucleic acid sequence encoding an extracellular domain, a transmembrane domain, a costimulatory signaling region, and a signaling domain of CD3-zeta, wherein the RNA is transcribed from an in vitro transcription vector or a DNA,

• wherein the in vitro transcription vector comprises the nucleic acid sequence of SEQ ID NO:
• 4, 5, 27, or 28, and
• wherein the DNA comprises the nucleic acid sequence of any one of SEQ ID NOs:
• 6-24.