Method for the enzymatic conversion of a phenol substrate into a corresponding catechol product
AssigneeUniversity College Dublin, National University of Ireland, Dublin
InventorsKevin O'Connor, Susan Molloy, Reeta Davis, Wesley Shaw
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Abstract
A method for the enzymatic conversion of a phenol substrate into a corresponding catechol product comprises the step of incubating the phenol substrate with a Ralstonia solanacearum tyrosinase enzyme, or a functional derivative thereof, in a reaction mixture, for a period of time sufficient to allow the enzyme convert at least some of the phenol substrate into the catechol product.
Claims13
A method for the enzymatic conversion of 4-halophenol into 4-halocatechol, the method comprising the step of incubating the 4-halophenol with a Ralstonia solanacearum tyrosinase enzyme or a functional derivative thereof, in a reaction mixture for a period of time sufficient to allow the Ralstonia solanacearum tyrosinase enzyme to convert at least 90% of the 4-halophenol into 4-halocatechol, in which the reaction mixture comprises ascorbic acid or its sodium salt, wherein the functional derivative is an engineered variant of Ralstonia solanacearum tyrosinase of SEQ ID NO:
- 1 selected from the group consisting of Y119F, V153A, D317Y and L330V (RV145);
- T183I, F185Y, N322S, and T359M (RVC10);
- and N322S (C10_N322S).
Dependencies are inferred from the claim text.