Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
AssigneeNovo Nordisk A/S
InventorsNicolaj Stroeyer Christophersen, Ulrik Doehn, Mattias Hansson
Life of this patent
Estimating the term…
Abstract
The present invention relates to generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors. The present invention also relates to functional beta cells produced by said methods and uses of said beta cells.
Claims14
A method for generating functional mature beta cells from endocrine progenitor cells, comprising the steps of (1) culturing the endocrine progenitor cells in a basal medium comprising a histone methyltransferase EZH2 inhibitor, a TGF-beta signaling pathway inhibitor, Heparin, and Nicotinamide, to obtain INS+ and NKX6.1+ double positive immature beta cells and (2) culturing the immature beta cells obtained in step (1) with 12% KOSR and GABA, to obtain the functional mature beta cells;
- wherein the endocrine progenitor cells are differentiated from pancreatic endoderm cells.
A method for generating INS+ and NKX6.1+ double positive immature beta cells from endocrine progenitor cells, comprising culturing the endocrine progenitor cells in a basal medium comprising a histone methyltransferase EZH2 inhibitor, a TGF-beta signaling pathway inhibitor, Heparin, and Nicotinamide;
- wherein the endocrine progenitor cells are differentiated from pancreatic endoderm cells.
Dependencies are inferred from the claim text.