# US 11,274,274 B2 · Inactivation of viruses

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,274,274 B2
- Document ID: US11274274B2
- Status: Granted
- Kind: B2
- Application number: 15/561,826
- Filed: 2016-04-12
- Published: 2018-05-03
- Granted: 2022-03-15
- Earliest claimed: 2015-04-20
- Assignees: Global Life Sciences Solutions USA LLC
- Applicants: Global Life Sciences Solutions USA LLC
- Inventors: Colin R. Tuohey; Richard Lee Damren; Thomas John Erdenberger; Parish M. Galliher; Patrick M. Guertin
- Web page: https://patentgrep.com/patent/US11274274B2

## CPC

- C12M 37/00: {Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination (C12M23/38 takes precedence; filtration in general and filters per se B01D24/00-B01D41/00; autoclaves B01J3/04; treatment of microorganisms with electrical or wave energy C12N13/00)}
- A61L 2/04: Heat (radiation A61L2/08)
- A61L 2/081: Gamma radiation
- C12M 23/14: {Bags}
- C12M 23/26: {flexible (flexible containers for laboratory use B01L3/505)}
- C12M 23/28: {disposable or single use}
- C12M 41/16: {by recirculation of culture medium at controlled temperature}
- C12M 41/22: {in contact with the bioreactor walls}
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- A61L 2/022: Filtration
- A61L 2103/05: Living organisms or biological materials
- A61L 2103/23: {Containers other than laboratory or medical, e.g. bottles or mail}
- A61L 2202/14: Means for controlling sterilisation processes, data processing, presentation and storage means, e.g. sensors, controllers, programs
- C12M 23/06: {Tubular (C12M23/08, C12M23/16 take precedence)}
- C12M 27/02: {Stirrer or mobile mixing elements}
- C12M 29/00: {Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B)}
- C12N 2795/00061: Methods of inactivation or attenuation
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The invention discloses a bioreactor apparatus (1;101;201;301) for cultivation of cells comprising: a) a disposable bioreactor vessel (2) with one or more walls (3,4,5) defining an inner volume (6), at least one port (10) in a wall, wherein the disposable bioreactor vessel is positioned in a rigid support structure (8;108); and b) a heater (9;109;209;309), capable of heating an amount of culture medium to a target temperature in the range of 55-95° C., while the amount of culture medium is being confined in or conveyed to the inner volume.

## Claims

### Claim 1

A bioreactor apparatus for cultivation of cells comprising:
a) a disposable bioreactor vessel with one or more walls defining an inner volume, at least one port in said one or more walls, wherein said disposable bioreactor vessel is positioned in a rigid support structure; and
b) a heater, capable of heating an amount of culture medium to a target temperature in the range of 55-95° C., said amount of culture medium being confined in or conveyed to said inner volume,
wherein said heater comprises a plastic or silicone disposable heater conduit fluidically-connected to said at least one port in said one or more walls of said disposable bioreactor vessel, and at least one heating element positioned to heat said disposable heater conduit,
wherein the at least one heating element is duct-shaped and at least one segment of said disposable heater conduit is confined within the duct-shaped heating element wherein the at least one segment of said disposable heater conduit contains one or more metallic structures and wherein the at least one segment is at least partially surrounded by two or more induction coils, wherein the two or more induction coils are in series.

### Claim 2 (depends on 1)

The bioreactor apparatus of claim 1, wherein said inner volume is at least 4 L.

### Claim 3 (depends on 1)

The bioreactor apparatus of claim 1, wherein said disposable bioreactor vessel is a flexible bag.

### Claim 4 (depends on 1)

The bioreactor apparatus of claim 1, wherein said disposable bioreactor vessel comprises at least one agitator in said inner volume.

### Claim 5 (depends on 1)

The bioreactor apparatus of claim 1, wherein said heater is capable of heating an amount of culture medium corresponding to at least 20%, at least 50% or at least 60% of said inner volume to a target temperature in the range of 55-95° C.

### Claim 6 (depends on 1)

The bioreactor apparatus of claim 1, wherein said rigid support structure has one or more walls defining an inner support volume and wherein said disposable bioreactor vessel has a shape conforming to, or essentially conforming to, a shape of said inner support volume.

### Claim 7 (depends on 1)

The bioreactor apparatus of claim 1, wherein said heater is capable of maintaining said amount of culture medium within an interval of the target temperature+/−5° C.

### Claim 8 (depends on 1)

The bioreactor apparatus of claim 1, wherein said one or more metallic structures comprise a ferromagnetic metal.

### Claim 9 (depends on 1)

The bioreactor apparatus of claim 1, wherein said one or more metallic structures has an axial length lmetal which is greater than an axial length lcoil of said two or more induction coils.

### Claim 10 (depends on 9)

The bioreactor apparatus of claim 9, wherein lmetal is at least 1.1 times greater than lcoil.

### Claim 11 (depends on 10)

The bioreactor apparatus of claim 10, further comprising a high-frequency alternating current power supply, electrically connected to said two or more induction coils.