Enveloped virus resistant to complement inactivation for the treatment of cancer
AssigneeWellstat ImmunoTherapeutics, LLC
InventorsTianci Luo, Rene Molina, Gabriel Castille
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Abstract
A recombinant fusion protein is disclosed. The fusion protein comprises: (a) a CD55 peptide sequence, (b) a linker sequence C-terminal to the CD55 sequence, (c) a transmembrane domain C-terminal to the linker sequence, and (d) an intracellular domain C-terminal to the transmembrane domain. The fusion protein does not contain a GPI anchor. The fusion protein can be expressed with an N-terminal secretory signal peptide, which is cleaved to yield the mature protein on the surface of a cell line or an enveloped virus. An oncolytic virus expressing the fusion protein is resistant to complement inactivation and can be used to treat cancer.
Claims15
A fusion protein comprising:
- (a) a CD55 peptide sequence comprising four short consensus repeats (SCR) of CD55, (b) a (G4S1)3 linker sequence C-terminal to the CD55 sequence, (c) a CD8 transmembrane domain C-terminal to the linker sequence, and (d) a CD8 intracellular domain C-terminal to the transmembrane domain wherein the CD8 intracellular domain is truncated;
- and wherein the fusion protein does not contain a GPI anchor.
Dependencies are inferred from the claim text.