Adenoviral coat protein derived delivery vehicles
AssigneesCentre National de la Recherche Scientifique (CNRS), European Molecular Biology Laboratory
InventorsImre Berger, Frédéric Garzoni, Pascal Fender
Life of this patent
Estimating the term…
Abstract
An engineered adenovirus penton base protomer, wherein the penton base protomer comprises a first RGD-loop, a second RGD-loop, a variable loop (V loop), an Adenovirus fiber protein binding cleft, and an N-terminal domain; wherein at least one of the first RGD-loop, the second RGD-loop, and the V loop comprises at least one inserted non-adenoviral antigenic polypeptide; and wherein the engineered adenovirus penton base protomer is capable of assembling into virus-like particles (VLPs).
Claims21
An engineered adenovirus penton base protomer, wherein the penton base protomer comprises a first RGD-loop, a second RGD-loop, a variable loop (V loop), an adenovirus fiber protein binding cleft, and an N-terminal domain;
- wherein at least one of the first RGD-loop, the second RGD-loop, and the V loop comprises at least one inserted non-adenoviral antigenic polypeptide;
- and
- wherein the engineered adenovirus penton base protomer is capable of assembling into virus-like particles (VLPs).
A method for producing a VLP, the method comprising:
- providing a recombinant host cell, the recombinant host cell comprising an expression vector, the expression vector comprising a nucleic acid encoding the engineered adenovirus penton base protomer;
- expressing the engineered adenovirus penton base protomer;
- purifying the engineered adenovirus penton base protomer;
- and
- allowing the purified engineered adenovirus penton base protomers to assemble into a VLP, the VLP comprising 12 pentamers, each of the 12 pentamers comprising five engineered adenovirus penton base protomers.
Dependencies are inferred from the claim text.