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US 11,268,962 B2Granted
Device for pretreatment of sample and analyzer equipped therewith, and method for pretreatment of sample
AssigneeShimadzu Corporation
InventorsTomoko Yamaguchi, Natsuki Iwata
Life of this patent
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Abstract
A sample is dissolved in a reagent 4 containing an organic solvent in a conversion vessel 11. A gas is supplied from a first gas supply part into the conversion vessel 11 via a reagent introduction tube 17, and thus the interior of the conversion vessel 11 is pressurized. A gas is supplied from a second gas supply part into the reagent 4 in the conversion vessel 11 via a reagent discharge tube 18, and thus gas bubbles 41 are formed in the reagent 4.
Claims5
A method for pretreatment of a sample comprising:
- a dissolution step of introducing a reagent containing an organic solvent into a vessel, and dissolving the sample in the reagent to form a sample-containing-reagent;
- a sample supply step of subjecting a protein to an Edman degradation, obtaining an amino acid from the protein by the Edman degradation, and introducing the obtained amino acid as the sample into the vessel;
- a gas supply step of supplying a first gas into the vessel outside the sample-containing-reagent, and thus pressurizing an interior of the vessel, and supplying a second gas into the sample-containing-reagent in the vessel and thus forming a gas bubble in the sample-containing-reagent to volatilize the sample-containing-reagent while the first gas is supplied to put an inside of the vessel in a high pressure state;
- and
- an analyzing step of analyzing the sample after the gas supply step,
- wherein the first gas and the second gas supplied by the gas supply step are an inert gas, and
- wherein a pressure of the first gas supplied from the gas supply step and a pressure of the second gas supplied from the gas supply step are set to values different from each other.
4 more elements
Dependencies are inferred from the claim text.