Microfluidic valve and a chip or system comprising the microfluidic valve

A microfluidic chip, comprising:

• a chip body, and
• one or more microfluidic valve disposed on the chip body, wherein the microfluidic valve comprises:
• a base;
• a sleeve disposed on the base;
• a rotor disposed within the sleeve located at the end of the sleeve away from the base, wherein the rotor, the sleeve, and the base form a mixing chamber;
• an aperture in the base capable of communicating or configured to communicate with the mixing chamber;
• and
• a micro-column disposed on the rotor and located in the mixing chamber, wherein the micro-column is capable of blocking or closing or configured to block or close the communication between the aperture and the mixing chamber.

A system, comprising one or more microfluidic chip and a means for detecting a reaction in the microfluidic chip,

• wherein the microfluidic chip comprises a chip body and one or more microfluidic valves disposed on the chip body, where each microfluidic valve comprises:
• a base;
• a sleeve disposed on the base;
• a rotor disposed within the sleeve located at the end of the sleeve away from the base, wherein the rotor, the sleeve, and the base form a mixing chamber;
• an aperture in the base capable of communicating or configured to communicate with the mixing chamber;
• and
• a micro-column disposed on the rotor and located in the mixing chamber, wherein the micro-column is capable of blocking or closing or configured to block or close the communication between the aperture and the mixing chamber.

A kit, comprising one or more microfluidic chip, and one or more reagents for performing a reaction in the microfluidic chip,

• wherein the microfluidic chip comprises a chip body and one or more microfluidic valves disposed on the chip body, where each microfluidic valve comprises:
• a base;
• a sleeve disposed on the base;
• a rotor disposed within the sleeve located at the end of the sleeve away from the base, wherein the rotor, the sleeve, and the base form a mixing chamber;
• an aperture in the base capable of communicating or configured to communicate with the mixing chamber;
• and
• a micro-column disposed on the rotor and located in the mixing chamber, wherein the micro-column is capable of blocking or closing or configured to block or close the communication between the aperture and the mixing chamber.

A method of using a microfluidic valve, wherein the microfluidic valve comprises:

• a base;
• a sleeve disposed on the base;
• a rotor disposed within the sleeve located at the end of the sleeve away from the base, wherein the rotor, the sleeve, and the base form a mixing chamber;
• an aperture in the base capable of communicating or configured to communicate with the mixing chamber;
• and
• a micro-column disposed on the rotor and located in the mixing chamber, wherein the micro-column is capable of blocking or closing or configured to block or close the communication between the aperture and the mixing chamber;
• and wherein the method comprises:
• 1) turning the rotor to open the aperture of the microfluidic valve;
• 2) introducing a liquid in the mixing chamber via the aperture;
• and
• 3) turning the rotor to stir or agitate the liquid in the mixing chamber to mix the substances in the liquid.