Carotenoid production method
AssigneeEneos Corporation
InventorsHidetada Nagai, Wataru Sato, Toshiyuki Takahashi, Harumi Sato
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Abstract
A mutant carotenoidogenic bacterium, comprising any of genes (a)-(c) below: (a) a gene encoding a protein comprising a mutant amino acid sequence in which at least the 225th amino acid residue in the amino acid sequence of 1-deoxy-D-xylulose 5-phosphate synthase of a carotenoidogenic bacterium has been substituted with other amino acid residue; (b) a gene encoding a protein comprising a mutant amino acid sequence in which at least the 305th amino acid residue in the amino acid sequence of decaprenyl diphosphate synthase of a carotenoidogenic bacterium has been substituted with other amino acid residue; and (c) both of the genes (a) and (b) above.
Claims11
A mutant carotenoidogenic bacterium that produces a carotenoid, comprising a combination of
- (i) a gene selected from (a)-(c), and
- (ii) a gene selected from (d)-(f);
- which are:
- (a) a gene encoding a protein comprising a mutant amino acid sequence in which at least glycine of the 225th amino acid residue in the amino acid sequence of SEQ ID NO:2 of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) of a carotenoidogenic bacterium belonging to the genus Paracoccus has been substituted with aspartic acid;
- (b) a gene encoding a protein comprising a mutant amino acid sequence in which glycine of the 225th amino acid residue in the amino acid sequence of SEQ ID NO:2 of DXS is substituted with aspartic acid and in which one to several amino acid residues other than the 225th amino acid residue have been deleted, substituted or added, and that has DXS activity;
- (c) a gene consisting of DNA comprising the nucleotide sequence of SEQ ID NO:5;
- (d) a gene encoding a protein comprising a mutant amino acid sequence in which at least alanine of the 305th amino acid residue in the amino acid sequence of SEQ ID NO:4 of decaprenyl diphosphate synthase (DPS) of a carotenoidogenic bacterium belonging to the genus Paracoccus has been substituted with valine;
- (e) a gene encoding a protein comprising a mutant amino acid sequence in which alanine of the 305th amino acid residue in the amino acid sequence of SEQ ID NO:4 of DPS is substituted with valine and in which one to several amino acid residues other than the 305th amino acid residue have been deleted, substituted or added, and that has reduced DPS activity;
- and
- (f) a gene consisting of DNA comprising the nucleotide sequence of SEQ ID NO:7.
A recombinant vector comprising a combination of
- (i) a gene selected from (a)-(c), and
- (ii) a gene selected from (d)-(f);
- which are:
- (a) a gene encoding a protein comprising a mutant amino acid sequence in which at least glycine of the 225th amino acid residue in the amino acid sequence of SEQ ID NO:2 of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) of a carotenoidogenic bacterium belonging to the genus Paracoccus has been substituted with aspartic acid;
- (b) a gene encoding a protein comprising a mutant amino acid sequence in which glycine of the 225th amino acid residue in the amino acid sequence of SEQ ID NO:2 of DXS is substituted with aspartic acid and in which one to several amino acid residues other than the 225th amino acid residue have been deleted, substituted or added, and that has DXS activity;
- (c) a gene consisting of DNA comprising the nucleotide sequence of SEQ ID NO:5;
- (d) a gene encoding a protein comprising a mutant amino acid sequence in which at least alanine of the 305th amino acid residue in the amino acid sequence of SEQ ID NO:4 of decaprenyl diphosphate synthase (DPS) of a carotenoidogenic bacterium belonging to the genus Paracoccus has been substituted with valine;
- (e) a gene encoding a protein comprising a mutant amino acid sequence in which alanine of the 305th amino acid residue in the amino acid sequence of SEQ ID NO:4 of DPS is substituted with valine and in which one to several amino acid residues other than the 305th amino acid residue have been deleted, substituted or added, and that has reduced DPS activity;
- and
- (f) a gene consisting of DNA comprising the nucleotide sequence of SEQ ID NO:7.
Dependencies are inferred from the claim text.