PHA-producing microorganism in which glycerol kinase activity is enhanced, and PHA production method using same
AssigneeKaneka Corporation
InventorsRina Aoki, Shunsuke Sato
Life of this patent
Estimating the term…
Abstract
Provided are a PHA-producing microorganism producing a higher molecular weight PHA and a PHA production method using the PHA-producing microorganism. A PHA-producing microorganism including a gene encoding a PHA synthase derived from genus Aeromonas, in which at least a portion of a PHA degrading enzyme gene is altered by substitution, deletion, insertion, and/or addition to reduce or eliminate activity of a PHA degrading enzyme encoded by the gene, and further a glycerol kinase activity is enhanced.
Claims14
A microorganism capable of producing a polyhydroxyalkanoate (PHA), comprising a gene encoding a PHA synthase derived from Aeromonas caviae,
- wherein at least a portion of a PHA degrading enzyme gene of the microorganism is altered by substitution, deletion, insertion, and/or addition of at least one nucleotide such that an activity of a PHA degrading enzyme encoded by the PHA degrading enzyme gene is eliminated or reduced as compared to an activity of the PHA degrading enzyme of a host of the microorganism, and
- a glycerol kinase activity of the microorganism is enhanced by introducing a gene encoding exogenous glycerol kinase as compared to a glycerol kinase activity of a host of the microorganism, wherein the gene encoding exogenous glycerol kinase is derived from Escherichia coli.
Dependencies are inferred from the claim text.