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US 11,268,083 B2Granted
Glucose isomerases
AssigneeMetgen Oy
InventorsKlara Birikh, Anu Minna Maaret Suonpää
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Abstract
The invention is in the field of enzymology. More in particular, it provides a method for the isomerization of glucose into fructose wherein the glucose is derived from lignocellulosic material. More in particular, the invention provides polypeptides encoding mutant glucose isomerase enzymes with improved glucose isomerase activity as compared to the corresponding wild type enzyme. The disclosed polypeptides are particularly suited for converting glucose to fructose in the presence of xylose.
Claims15
A polypeptide with glucose isomerase activity, wherein the polypeptide comprises:
- an amino acid sequence that has at least 90% sequence identity to the full length of the amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2,
- wherein the polypeptide comprises a Glycine, Serine, Alanine or Cysteine residue at a position corresponding to position 104 in SEQ ID NO:
- 1 or SEQ ID NO:
- 2.
3 more elements
Dependencies are inferred from the claim text.