Afucosylated protein, cell expressing said protein and associated methods

A method of obtaining an antibody producing cell, said method comprising transfecting a cell comprising a gene encoding an antibody having ACDD activity with a vector comprising:

• a nucleotide sequence encoding a crRNA consisting of SEQ ID NO:12,
• a nucleotide sequence encoding a crRNA consisting of SEQ ID NO:14,
• a nucleotide sequence encoding tracrRNA, and
• a nucleotide sequence encoding a Cas9n nuclease, wherein the nucleotide sequence encoding the Cas9n nuclease consists of SEQ ID No. 3 to obtain the antibody producing cell.

A method of obtaining an afucosylated antibody having ADCC activity, said method comprising obtaining an antibody expressed by an antibody producing cell, said antibody producing cell comprising:

• a gene encoding an antibody having ADCC activity, and
• a vector comprising:
• a nucleotide sequence encoding a crRNA consisting of SEQ ID No. 12,
• a nucleotide sequence encoding a crRNA consisting of SEQ ID No. 14,
• a nucleotide sequence encoding tracrRNA, and
• a nucleotide sequence encoding a Cas9n nuclease, wherein the nucleotide sequence encoding the Cas9n nuclease consists of SEQ ID No. 3.