Method of painting micro vesicles
AssigneeVin De Bona Trading Company Pte Ltd
InventorsJohn Dangerfield, Eva Maria Brandtner, Wee Jin Tan, Christoph Metzner
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Abstract
The present invention relates to a method to modify and/or to isolate exosomes and other naturally occurring plasma membrane derived microvesicles, by incubation with a reactant, consisting of at least a membrane anchor domain or moiety and a hydrophilic functional domain or moiety. The invention also relates to modification using the same of artificially-prepared lipid bilayer vesicles called liposomes (composed of natural phospholipids) and non-biological or “synthetic” block copolymer membrane mimics which also form vesicles in aqueous solution called polymersomes.
Claims15
A method for exogenously modifying the membrane composition of a membrane microvesicle (MMV), comprising
- incubating an aqueous sample comprising MMV with a reactant comprising a hydrophilic target domain or moiety covalently linked to a lipophilic membrane anchor domain or moiety, wherein the lipophilic membrane anchor domain becomes integrated into the lipid double layer of the membrane and wherein the hydrophilic target domain becomes exposed to the aqueous sample fluid,
- wherein the hydrophilic target domain of the reactant carries one or more extraction (EX)-tags.
A method for isolating, enriching or concentrating of MMV, comprising the steps
- (a) incubating an aqueous sample comprising MMV with a reactant comprising a hydrophilic target domain or moiety covalently linked to a lipophilic membrane anchor domain or moiety, wherein the lipophilic membrane anchor domain becomes integrated into the lipid double layer of the membrane thereby providing membrane modified MMV, wherein the hydrophilic target domain becomes exposed to the surrounding aqueous sample fluid, and wherein the hydrophilic target domain of the reactant carries an EX-tag, and
- (b) isolating membrane modified MMV from the sample fluid and/or excessive reactants, with the use of said tag.
Dependencies are inferred from the claim text.