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US 11,266,693 B2Granted
Nucleic acids encoding engineered meganucleases with recognition sequences found in the human T cell receptor alpha constant region gene
AssigneePrecision BioSciences, Inc.
InventorsMichael G. Nicholson, James Jefferson Smith, Derek Jantz, Victor Bartsevich, Daniel T. MacLeod, Jeyaraj Antony
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Abstract
Disclosed herein are recombinant meganucleases engineered to recognize and cleave a recognition sequence present in the human T cell receptor alpha constant region gene. The present disclosure further relates to the use of such recombinant meganucleases in methods for producing genetically-modified eukaryotic cells.
Claims9
An isolated polynucleotide comprising a nucleic acid sequence encoding an engineered meganuclease, wherein said engineered meganuclease binds and cleaves at a recognition sequence consisting of SEQ ID NO:
- 3, wherein said engineered meganuclease comprises a first subunit and a second subunit, wherein said first subunit binds to a first recognition half-site of said recognition sequence, and wherein said second subunit binds to a second recognition half-site of said recognition sequence, and wherein said engineered meganuclease comprises an amino acid sequence having at least 96% identity to SEQ ID NO:
- 8.
Dependencies are inferred from the claim text.