Method and system for detecting aromatic compounds

A method of analyzing aromatic compounds in a sample, comprising:

• preparing a diazonium reagent;
• contacting the aromatic compounds in the sample with the diazonium reagent to form a first analyte;
• measuring an amount or ratio of the first analyte to the sample that has been formed by liquid chromatography-mass spectrometry analyzing;
• and
• extrapolating presence or quantity of the aromatic compounds in the sample based on the measured amount or ratio of the first analyte to the sample that has been formed;
• wherein the contacting comprises having azo coupling reaction between the diazonium reagent and the aromatic compounds, and the analyte is an azo-coupled analyte;
• wherein the diazonium reagent contains one or more functional groups that are negatively or positively charged during liquid chromatography-mass spectrometry analyzing;
• the functional groups that are negatively charged in the diazonium reagent comprise one or more of sulfonate group (—SO3−), carboxylate group (—COO−), sulfonic acid group (—SO3H) and carboxylic acid group (—COOH), and the functional groups that are positively charged in the diazonium reagent include one or more of quaternary ammonium group, amino group and thiol group;
• wherein the diazonium reagent is p-sulfanilic acid diazonium salt solution, Fast Red RC Diazonium Salt or Fast Red TR Diazonium Salt;
• when the diazonium reagent is p-sulfanilic acid diazonium salt solution, the aromatic compounds in the sample is Estriol or Estradiol;
• and
• when the diazonium reagent is Fast Red RC Diazonium Salt or Fast Red TR Diazonium Salt, the aromatic compounds in the sample is cannabidiol compounds, Estriol or Estradiol.