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US 11,262,297 B2Granted
Substrate for fluorescence amplification
AssigneesFianostics GmbH, Stratec Consumables GmbH
InventorsChristoph Mauracher, Georg Bauer, Adrian Prinz, Gottfried Aichinger, Gerhard Hawa
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Abstract
The present invention relates to the use of a substrate for enhancing the fluorescence of a fluorescent molecule, wherein the substrate comprises a solid polymer carrier having a plurality of recesses separated from each other and wherein the solid carrier is coated at least in part by a metal.
Claims22
A method for enhancing the fluorescence of one or several fluorescent molecules,
- comprising:
- providing a substrate that comprises a solid polymer carrier having a plurality of recesses separated from each other,
- wherein the recesses in the plurality of recesses have a depth of 0.1 μm to 5 μm,
- wherein the solid polymer carrier is coated at least in part by at least one metal layer comprising silver or an alloy thereof,
- wherein the at least one metal layer has a thickness ranging from 10 nm to 60 nm;
- positioning the one or several fluorescent molecules in a spatial proximity of the substrate coated at least in part by the at least one metal layer;
- and
- exciting the one or several fluorescent molecules with light at a suitable wavelength,
- thereby enhancing the fluorescence of the one or several fluorescent molecules.
6 more elements
A method for quantifying at least one analyte in a sample, comprising:
- a) directly or indirectly labelling the at least one analyte with at least one fluorophore to produce at least one labelled analyte,
- b) applying the at least one labelled analyte onto a substrate that comprises
- a solid polymer carrier having a plurality of recesses separated from each other,
- wherein the recesses in the plurality of recesses have a depth of 0.1 μm to 5 μm,
- wherein the solid polymer carrier is coated at least in part by at least one metal layer comprising silver or an alloy thereof,
- wherein the at least one metal layer has a thickness ranging from 10 nm to 60 nm;
- wherein the at least one fluorophore is positioned in a spatial proximity of the solid polymer carrier coated at least in part by the at least one metal layer;
- c) exciting the at least one fluorophore by irradiation of the substrate using light at an appropriate wavelength, and
- d) measuring the fluorescence from the at least one fluorophore to quantify the at least one analyte in the sample.
6 more elements
Dependencies are inferred from the claim text.