Promoters

An expression cassette comprising a promoter sequence selected from the group consisting of sequences having at least 95% sequence identity and/or homology to SEQ ID NO. 1 (4pgG600), SEQ ID NO. 2, (4pMCP600), SEQ ID NO. 3 (p430), SEQ ID NO:

• 4 (p455), and the complementary nucleotide sequences thereof,
• wherein the promoter sequence is operably linked to a nucleotide sequence of interest,
• wherein said promoter sequence leads to expression of the nucleotide sequence of interest,
• whereby said promoter sequence is a heterologous promoter sequence, and/or an exogenous promoter sequence.

An eukaryotic host cell line comprising a vector comprising a promoter sequence selected from the group consisting of sequences having at least 95% sequence identity and/or homology to SEQ ID NO. 1 (4pgG600), SEQ ID NO. 2 (4pMCP600), SEQ ID NO. 3 (p430), SEQ ID NO:

• 4 (p455), and the complementary nucleotide sequences thereof,
• wherein the promoter sequence is operably linked to a nucleotide sequence of interest, selected from the group consisting of:
• a gene of interest, a heterologous and/or exogenous sequence of interest, or an antigen encoding sequence of interest,
• wherein said promoter sequence leads to expression of the nucleotide sequence of interest,
• whereby said promoter sequence is a heterologous promoter sequence, and/or an exogenous promoter sequence,
• wherein said host cell line is a mammalian cell line or an insect cell line, selected from the group consisting of:
• a PK/WRL cell line, a RK13 cell line, a MDBK cell line, a ST cell line, an AI-ST cell line, a VERO cell line, a Sf9 cell line, a Sf21, a Sf plus cell line, a MDCK cell line, and/or derivatives thereof.

A kit comprising

• a. a host cell(s),
• b. optionally a transfection reagent(s),
• c. an instruction leaflet, and
• d. a vector comprising a promoter sequence selected from the group consisting of sequences having at least 95% sequence identity and/or homology to SEQ ID NO. 1 (4pgG600), SEQ ID NO. 2 (4pMCP600), SEQ ID NO. 3 (p430), SEQ ID NO:
• 4 (p455), and the complementary nucleotide sequences thereof,
• wherein the promoter sequence is operably linked to a nucleotide sequence of interest, selected from the group consisting of:
• a gene of interest, a heterologous and/or exogenous sequence of interest, or antigen encoding sequence of interest,
• wherein said promoter sequence leads to expression of the nucleotide sequence of interest,
• whereby said promoter sequence is a heterologous promoter sequence, and/or an exogenous promoter sequence.

A method of producing a vector, comprising

• a. providing a promoter sequence comprising SEQ ID NO:
• 1 (4pgG600), SEQ ID NO. 2 (4pMCP600), SEQ ID NO. 3 (p430), SEQ ID NO:
• 4 (p455), or the complementary nucleotide sequences thereof, wherein said promoter sequence leads to expression of a nucleotide sequence of interest, and/or an antigen encoding sequence of interest,
• b. integrating said promoter sequence of step a) into a vector backbone derived from a virus, which is selected from the group consisting of:
• Herpesviridae, varicelloviruses, Adenoviridae (AdV), Parvoviridae like Adena-associated viruses, Baculoviridae, Retroviridae, and Poxviridae.

A method of preparing a host cell, comprising:

• a) infecting a permissive eukaryotic host cell line with a vector comprising a promoter sequence selected from the group consisting of SEQ ID NO:
• 1 (4pgG600), SEQ ID NO:
• 2 (4pMCP600), SEQ ID NO:
• 3 (p430), SEQ ID NO:
• 4 (p455), and the complementary nucleotide sequences thereof and a functional and the complementary nucleotide sequences thereof,
• wherein the promoter sequence is operably linked to a nucleotide sequence of interest, selected from the group consisting of:
• a gene of interest, a heterologous and/or exogenous sequence of interest, or antigen encoding sequence of interest,
• wherein said promoter sequence leads to expression of the nucleotide sequence of interest, whereby said promoter sequence is a heterologous promoter sequence, and/or an exogenous promoter sequence,
• b) cultivating the infected cells of a) under suitable conditions, and
• c) optionally harvesting said host cell.

A method for the preparation of an immunogenic composition or a vaccine for reducing the incidence or the severity of one or more clinical signs associated with or caused by an infection, comprising the following steps:

• a) infecting a permissive eukaryotic host cell line with a vector comprising a promoter sequence selected from the group consisting of SEQ ID NO:
• 1 (4pgG600), SEQ ID NO. 2 (4pMCP600), SEQ ID NO. 3 (p430), SEQ ID NO:
• 4 (p455), and the complementary nucleotide sequences thereof and a functional and the complementary nucleotide sequences thereof,
• wherein the promoter sequence is operably linked to a nucleotide sequence of interest, selected from the group consisting of:
• a gene of interest, a heterologous and/or exogenous sequence of interest, or antigen encoding sequence of interest,
• wherein said promoter sequence leads to expression of the nucleotide sequence of interest,
• whereby said promoter sequence is a heterologous promoter sequence, and/or an exogenous promoter sequence,
• b) cultivating the infected cells of a) under suitable conditions,
• c) harvesting infected cells of b) and/or vector and/or virus components,
• d) optionally purifying the harvest of step c), and
• e) admixing said harvest with a pharmaceutically acceptable carrier.