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US 11,261,455 B2Granted
Sugarcane mosaic virus as a transient gene expression vector
AssigneeIowa State University Research Foundation, Inc.
InventorsJohn Hemmingson Hill, Steven Alan Whitham, Yu Mei, Chunquan Zhang
Life of this patent
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Abstract
The present invention provides plant virus vectors developed from the Sugarcane mosaic virus (SCMV). The vectors include a nucleic acid sequence encoding an infectious Sugarcane mosaic virus (SCMV) operably linked to one or more regulatory elements functional in a plant. The plant virus vectors may be used to infect monocot plants, such as maize, for gene expression applications.
Claims21
A vector for expression of a foreign nucleotide sequence in a plant comprising:
- a nucleic acid molecule encoding an infectious Sugarcane mosaic virus (SCMV) operably linked to one or more regulatory elements functional in a plant cell, wherein the SCMV is at least 95% identical to the sequence set forth in SEQ ID NO:
- 16, 17, or 18.
A method of expressing a foreign nucleotide sequence of interest in a plant cell comprising:
- introducing to said plant cell a vector comprising a nucleic acid molecule encoding an infectious SCMV operably linked to one or more regulatory elements functional in a plant cell, wherein the SCMV is at least 95% identical to the sequence set forth in SEQ ID NO:
- 16, 17, or 18, and wherein the SCMV has the foreign nucleotide sequence inserted therein.
Dependencies are inferred from the claim text.