Method of separating lipids from a lysed lipids containing biomass

A method of separating polyunsaturated fatty acids (PUFAs) containing lipid from the debris of a biomass, comprising the steps:

• a) providing a suspension of a biomass comprising cells which contain a PUFAs containing lipid;
• b) lysing the cells of the biomass;
• c) adding acetone to the suspension obtained in step (b) until a final amount of between 25 and 47.5 wt.-% of acetone is reached;
• d) thoroughly mixing the suspension obtained in step (c);
• e) separating an oil containing PUFAs and acetone-containing light phase obtained in step (d) from a water, acetone, salt and cell debris containing heavy phase.