Cell surface coupling of nanoparticles
AssigneeMassachusetts Institute of Technology (Mitn1)
InventorsDarrell J. Irvine, Yiran Zheng, Li Tang
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Abstract
The present disclosure is directed, in some embodiments, to methods and compositions of comprising a cell having a non-internalizing receptor, and a nanoparticle surface-modified with a ligand that binds to the non-internalizing receptor.
Claims20
A method of treating a tumor in a subject, the method comprising administering to the subject a composition comprising:
- (i) a nucleated carrier cell that homes to a tumor and expresses CD45 and a cytokine receptor on its surface;
- and
- (ii) a protein nanogel comprising cytokines that interact with said cytokine receptor, wherein the cytokines are reversibly and covalently crosslinked to each other through a degradable linker,
- wherein the nanogel is coupled to the surface of the nucleated carrier cell with an anti-CD45 monoclonal antibody, thereby treating cancer.
A method of treating a tumor in a subject, the method comprising administering to the subject a composition comprising:
- (i) a nucleated carrier cell that homes to a tumor and expresses CD45 and an IL-2 receptor on its surface;
- and
- (ii) a protein nanogel comprising IL-2 polypeptides reversibly and covalently crosslinked to each other through a degradable linker,
- wherein the nanogel is coupled to the surface of the nucleated carrier cell with an anti-CD45 monoclonal antibody, thereby treating cancer.
A method of treating a tumor in a subject, the method comprising administering to the subject a composition comprising:
- (i) a nucleated carrier cell that homes to a tumor and expresses CD45 and an IL-15 receptor on its surface;
- and
- (ii) a protein nanogel comprising IL-15 polypeptides reversibly and covalently crosslinked to each other through a degradable linker,
- wherein the nanogel is coupled to the surface of the nucleated carrier cell with an anti-CD45 monoclonal antibody, thereby treating cancer.
Dependencies are inferred from the claim text.