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US 11,254,971 B2Granted
Nucleic acid amplification and detection assays
AssigneeCambridge Molecular Diagnostics Ltd
InventorGustavo Andrés Cerda-Moya
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Abstract
The present invention relates to a method and kit for amplifying and detecting a quantity of nucleic acid. The invention is particularly relevant to isothermal amplification techniques carried out on a flow based assay device. The amplified nucleic acid may be detected on the device using an optical read-out.
Claims18
A test strip device for detecting the presence of a target analyte in a fluid applied to the device, the device comprising:
- i) a displacement area having a first immobilised marker which can be displaced by the presence of the target analyte to produce a first released marker, wherein the first released marker comprises a viral protease;
- ii) a signal amplification area having further markers or detectable markers immobilised via a peptide which is specifically cleaved by the presence of the viral protease;
- iii) optionally one or more further signal amplification areas having further immobilised markers which are released by the presence of a released marker, wherein one or more of the signal amplification areas releases a detectable marker;
- and
- iv) one or more detection areas which can identify the presence of the detectable marker;
- wherein the displacement area, signal amplification area(s) and detection area(s) are connected on a porous material such that fluid can flow from the displacement area through the signal amplification area(s) and into the detection area(s) upon application of the fluid to the device.
3 more elements
A method of detecting a target analyte, the method comprising applying a sample to a test strip device having
- i) a displacement area having a first immobilised marker which can be displaced by the presence of the target analyte to produce a first released marker, wherein the first released marker comprises a viral protease;
- ii) a signal amplification area having further markers or detectable markers immobilised via a peptide which is specifically cleaved by the presence of the viral protease;
- iii) optionally one or more further signal amplification areas having further immobilised markers which are released by the presence of a released marker, wherein one or more of the signal amplification areas releases a detectable marker;
- and
- iv) one or more detection areas which can identify the presence of the detectable marker;
- wherein the displacement area, signal amplification area(s) and detection area(s) are connected on a porous material such that fluid can flow from the displacement area through the signal amplification area(s) and into the detection area(s);
- flowing the sample from the displacement area through the signal amplification area(s) and into the detection area(s) and determining from the presence of the detectable marker in the detection area the presence of the target analyte in the sample.
4 more elements
Dependencies are inferred from the claim text.