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AssigneesHocusLocus, LLC, Universität fur Bodenkulture
InventorsEdward Eveleth, Nicole Borth, Scott A. Tenenbaum, Francis J. Doyle, II, Zachary Edward Wurz
Estimating the term…
Described herein are methods and compositions for producing a gene of interest (GOI) which, in certain embodiments, can reduce the metabolic burden on cells and reduce decoupling of GOI production from marker production, as compared to prior art methods. The methods relate to positive selection and negative selection approaches to establishing high GOI-producing cell lines, e.g., CHO lines. In certain embodiments, the methods comprise transfecting a cell with (a) an oligonucleotide comprising a GOI and a non-coding RNA, and (b) an oligonucleotide encoding a selection protein; wherein the non-coding RNA promotes or inhibits production of the selection protein. The cell producing the GOI can be identified and/or selected as a result of or by detecting the absence or the presence of the selection protein.
A method for identifying a cell producing a protein encoded by a gene of interest (GOI), the method comprising:
A method for promoting survival of cells producing a protein encoded by a gene of interest (GOI), the method comprising:
A method for identifying a cell producing a protein encoded by a gene of interest (GOI), the method comprising:
Dependencies are inferred from the claim text.