ADAMTS13 protein cell culture supernatant
AssigneeTakeda Pharmaceutical Company Limited
InventorsLeopold Grillberger, Alexandra Spenger, Meinhard Hasslacher, Rana Grillberger, Manfred Reiter
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Abstract
The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.
Claims22
A cell culture supernatant, the supernatant comprising recombinant human ADAMTS13 protein,
- wherein the supernatant comprises a specific activity of at least 600 units of ADAMTS13 activity per mg of recombinant human ADAMTS13 protein, as measured using a FRETS-VWF73 assay, and wherein the supernatant comprises at least 3 μM zinc.
Dependencies are inferred from the claim text.