Systems and methods for optically processing samples
AssigneeBioelectronica Corporation
InventorsLeonard Sherman, Roger Chen, Jonathan F. Hull
Life of this patent
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Abstract
A system for processing a sample includes a chamber having at least one inlet and at least one outlet, where the chamber is configured to accommodate flow of the sample from the at least one inlet toward the at least one outlet, and an imager array configured to image the flow of the sample in the chamber, where the imager array includes at least one lensless image sensor configurable opposite at least one light source.
Claims10
A mixture comprising:
- an encapsulation reagent;
- one or more first particles suspended in aqueous media, each first particle comprising a first binding partner;
- and
- a population of cells, comprising at least one cell of interest that secretes a protein of interest having a second binding partner, wherein the first binding partner is specific to the second binding partner.
A method of preparing a sample for a clustering assay system, the method comprising:
- providing a population of cells, the population of cells comprising at least one cell of interest;
- combining the population of cells, a first plurality of particles, and an encapsulation reagent to create a mixture, wherein each particle of the first plurality of particles is suspended in aqueous media and comprises a first binding partner that is specific to a second binding partner secreted by the at least one cell of interest;
- and
- agitating the mixture to create an emulsion, thereby encapsulating the population of cells into a plurality of polydisperse sample entities.
A method of selecting at least one cell of interest from a population of cells, the method comprising:
- providing an emulsion comprising the population of cells and a first plurality of particles, wherein the population of cells and the first plurality of particles are encapsulated into a plurality of polydisperse sample entities, and wherein each particle of the first plurality of particles is suspended in aqueous media and comprises a first binding partner that is specific to a second binding partner secreted by the at least one cell of interest;
- measuring a signal for at least one sample entity, wherein the signal is at least partially associated with binding of the first and second binding partners;
- and
- identifying the at least one cell of interest based at least in part on the measured signal.
Dependencies are inferred from the claim text.