Method for producing cellulase and apparatus for said method
AssigneeToray Industries, Inc.
InventorsHiroyuki Kurihara, Katsushige Yamada
Life of this patent
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Abstract
A method of producing cellulase includes steps (1) to (3): (1) subjecting an aqueous solution of cellulase derived from filamentous fungi to filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 to 200,000 to obtain a filtrate and concurrently obtain a concentrated enzyme liquid as a retentate; (2) further subjecting the filtrate obtained in step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 to 50,000 to obtain a second concentrated enzyme liquid as a retentate; and (3) mixing the concentrated enzyme liquid obtained in steps (1) and (2) to obtain cellulase derived from filamentous fungi.
Claims18
A method of producing cellulase comprising a microcrystalline cellulose degrading activity, comprising steps (1) to (3):
- (1) subjecting an aqueous solution of cellulase derived from filamentous fungi to liquid-liquid filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 Da to 200,000 Da to obtain a filtrate and concurrently obtain a concentrated enzyme solution as a retentate;
- (2) further subjecting the filtrate obtained in step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 Da to 50,000 Da to obtain a second concentrated enzyme solution as a retentate;
- and
- (3) mixing the concentrated enzyme solution obtained in steps (1) and (2) to obtain cellulase comprising a microcrystalline cellulose degrading activity, derived from filamentous fungi.
A method of producing cellulase comprising a microcrystalline cellulose degrading activity, comprising
- an ultrafiltration step consisting of steps (1) and (2),
- (1) subjecting an aqueous solution of cellulase derived from filamentous fungi to liquid-liquid filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 Da to 200,000 Da to obtain a filtrate and concurrently obtain a concentrated enzyme solution as a retentate;
- (2) further subjecting the filtrate obtained in step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 Da to 50,000 Da to obtain a second concentrated enzyme solution as a retentate;
- and
- (3) mixing the concentrated enzyme solution obtained in steps (1) and (2) to obtain cellulase comprising a microcrystalline cellulose degrading activity, derived from filamentous fungi.
Dependencies are inferred from the claim text.