Production of mogroside compounds in recombinant hosts
AssigneeEvolva SA
InventorJens Houghton-Larsen
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Abstract
The invention relates to recombinant microorganisms and methods for producing mogroside compounds and mogroside precursors.
Claims24
A recombinant host cell capable of producing one or more mogroside compounds in a cell culture, the host cell comprising a recombinant gene encoding a polypeptide capable of deglycosylating a mogroside precursor and having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:2;
- wherein the polypeptide:
- (a) comprises a catalytically active portion of an endogenous glucosidase polypeptide or an endogenous glucanase polypeptide;
- (b) does not comprise a signal peptide or a transmembrane domain that is comprised by the endogenous glucoside polypeptide;
- and
- (c) is free of a domain facilitating secretion of the polypeptide from the host cell;
- wherein the host cell is capable of retaining at least about 50% of the expressed polypeptide capable of deglycosylating the mogroside precursor in a cytosol of the host cell;
- wherein the one or more mogroside compounds are a deglycosylation product of the mogroside precursor;
- and
- wherein expression of the gene increases production of the one or more mogroside compounds.
A method of producing one or more mogroside compounds in a cell culture, comprising culturing a recombinant host cell capable of producing the one or more mogroside compounds in the cell culture, the host cell comprising a recombinant gene encoding a polypeptide capable of deglycosylating a mogroside precursor and having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:2 in the cell culture, under conditions in which the genes are expressed;
- wherein the polypeptide:
- (a) comprises a catalytically active portion of an endogenous glucosidase polypeptide or an endogenous glucanase polypeptide;
- (b) does not comprise a signal peptide or a transmembrane domain that is comprised by the endogenous glucoside polypeptide;
- and
- (c) is free of a domain facilitating secretion of the polypeptide from the host cell;
- wherein the host cell is capable of retaining at least about 50% of the expressed polypeptide capable of deglycosylating the mogroside precursor in a cytosol of the host cell;
- wherein expression of the gene increases production of the one or more mogroside compounds;
- wherein the one or more mogroside compounds are produced by the recombinant host cell;
- and
- wherein the one or more mogroside compounds are a deglycosylation product of the mogroside precursor.
Dependencies are inferred from the claim text.