Methods and systems of producing oligosaccharides
AssigneeCambridge Glycoscience Ltd
InventorThomas Simmons
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Abstract
The present invention in the field of oligosaccharide production provides a method of producing oligosaccharides of useful lengths without producing substantial amounts of monosaccharides and disaccharides (illustrated by FIG. 1). There is provided a method for producing an ingredient suitable for incorporation into a foodstuff, cosmetic, or nutraceutical, said ingredient comprising one or more oligosaccharides, wherein the oligosaccharides are produced in an enzymatic reaction, said enzymatic reaction comprising the step of contacting, in a solution or suspension, a polysaccharide-cleaving enzyme and a polysaccharide-containing feedstock, wherein said enzymatic reaction produces substantially no monosaccharides or disaccharides.
Claims27
A method for producing a sugar substitute, the method comprising:
- (a) contacting, in a solution or suspension, more than one type of polysaccharide-cleaving enzyme and a polysaccharide-containing feedstock to perform one or more enzymatic reactions to provide a reacted feedstock having less than 20% w/w monosaccharides;
- and
- (b) filtering (i) more than one type of oligosaccharide and (ii) at least one type of monosaccharide from the reacted feedstock of step (a) to produce the sugar substitute, such that the sugar substitute comprises less than 20% w/w monosaccharides and less than 60% w/w disaccharides,
- wherein the more than one type of polysaccharide-cleaving enzyme comprises a xylanase.
Dependencies are inferred from the claim text.