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US 11,248,221 B2Granted
Agarase-3,6-anhydro-L-galactosidase-arabinose isomerase enzyme complex and method for production of tagatose from agar using the same
AssigneeKorea University Research and Business Foundation
InventorsSung Ok Han, Da-Woon Jeong, Jeong Eun Hyeon
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Abstract
The present disclosure relates to an enzyme complex of arabinose isomerase, agarase and 3,6-anhydro galactosidase and a method for producing tagatose by degrading agar using the same. By using the enzyme complex according to the present disclosure, agar obtained from marine biomass can be degraded effectively and useful physiologically active substances such as tagatose can be obtained effectively therefrom.
Claims15
An agarase complex comprising:
- a mini scaffold protein comprising a cohesin module;
- a first fusion protein in which a monosaccharide convertase and a first dockerin module are bound;
- a second fusion protein in which agarase and a second dockerin module are bound;
- and
- a third fusion protein in which 3,6-anhydro-L-galactosidase and a third dockerin module are bound,
- wherein the first, the second and the third fusion proteins are linked to the mini scaffold protein via dockerin-cohesin binding,
- wherein the monosaccharide convertase is arabinose isomerase derived from Lactobacillus, and
- wherein the arabinose isomerase comprises the amino acid sequence of SEQ ID NO 1.
5 more elements
A method for preparing an enzyme complex, comprising:
- preparing a first fusion protein by linking a first dockerin module to an arabinose isomerase;
- preparing a second fusion protein by linking a second dockerin module to a β-agarase;
- preparing a third fusion protein by linking a third dockerin module to a 3,6-anhydro-L-galactosidase;
- preparing a mini scaffold protein having a cohesin module;
- and
- preparing the enzyme complex by binding the cohesin module to the first, the second, and the third dockerin modules by quantifying the first, the second, and the third fusion proteins and the mini scaffold protein to a same concentration and proportion and mixing them in a binding solution comprising 25 mM calcium chloride (CaCl2)),
- wherein the arabinose isomerase comprises the amino acid sequence of SEQ ID NO 1.
4 more elements
Dependencies are inferred from the claim text.