Nucleic acid integrated detection method and detection reagent tube

A detection reagent tube for realizing a nucleic acid integrated detection method, the detection reagent tube comprising:

• a main tube and one or more branch tubes provided at a lower end of the main tube, wherein a lysing zone, a cleaning zone and a plurality of separation plugs comprising at least a least a separation plug (3) and a second separation plug (4) which are sequentially disposed one on top of another in the main tube, wherein a reaction zone is provided in each branch tube and the second separation plug is positioned at a connection between the one or more branch tubes and the main tube;
• a reagent storage chamber with a downward opening disposed in the second separation plug, a carrier for storing a reagent disposed in the reagent storage chamber and a hydrophobic sealing layer in the reagent storage chamber;
• hydrophobic layers in a liquid or a solid phase disposed at each of the plurality of separation plugs;
• and
• a magnetic bead channel for magnetically carrying the nucleic acid to sequentially pass through each hydrophobic layer penetrating to the one or more branch tubes defined in an inner wall of the main tube.