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US 11,242,547 B2Granted
Neurotransmitters and methods of making the same
AssigneePurissima, Inc.
InventorsGeorge Rudenko, Robert Evans
Life of this patent
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Abstract
In an aspect, the disclosure provides methods for making neurotransmitters in a host organism. The neurotransmitters can be cannabinoids and derivatives of cannabinoids. The host cells can be microalgae, fungi or other host cells. In a related aspect, the disclosure provides host cells engineered to have biochemical pathways for making neurotransmitters such as cannabinoids.
Claims20
A method, comprising the steps of:
- providing a Prototheca host cell wherein the Prototheca host cell comprises a first nucleic acid encoding SEQ ID NO:
- 1, a second nucleic acid encoding SEQ ID NO:
- 2, a third nucleic acid encoding SEQ ID NO:
- 3, and a fourth nucleic acid encoding SEQ ID NO:
- 4, wherein the first nucleic acid is operably linked to a control region, wherein the second nucleic acid is operably linked to a control region, wherein the third nucleic acid is operably linked to a control region, and wherein the fourth nucleic acid is operably linked to a control region, introducing the Prototheca cell into a media wherein the media comprises a carbon source, culturing the Prototheca cell in the media whereby a cannabinoid is made.
2 more elements
Dependencies are inferred from the claim text.