Display substrate, manufacturing method thereof, display panel, and display device

A display substrate comprising:

• a first substrate, a first sub-pixel layer and a second sub-pixel layer on the first substrate,
• wherein the first sub-pixel layer comprises a plurality of first sub-pixels, the second sub-pixel layer comprises a plurality of second sub-pixels, a first orthographic projection of the plurality of first sub-pixels on the first substrate and a second orthographic projection of the plurality of second sub-pixels on the first substrate do not overlap, and the first sub-pixel layer and the second sub-pixel layer are not on a same plane;
• a first spacing region is between the first sub-pixels, and a second spacing region is between the second sub-pixels;
• one of the plurality of first sub-pixels is arranged corresponding to the second spacing region, and one of the plurality of second sub-pixels is arranged corresponding to the first spacing region;
• a width of the first spacing region is larger than a width of the one of the plurality of second sub-pixels corresponding to the first spacing region;
• a width of the second spacing region is larger than a width of the one of the plurality of first sub-pixels corresponding to the second spacing region;
• the first sub-pixel layer and the second sub-pixel layer are sequentially located on a side of the first substrate in a direction perpendicular to the first substrate;
• a second substrate is between the first sub-pixel layer and the second sub-pixel layer, and
• a third substrate is between the first sub-pixel layer and the second substrate,
• wherein a pitch between the first sub-pixel and the second sub-pixel adjacent to the first sub-pixel in a direction parallel to the first substrate is half of a pixel interval between the first sub-pixels in the first sub-pixel layer and half of a pixel interval between the second sub-pixels in the second sub-pixel layer.