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US 11,239,066 B2Granted
Cell population analysis
AssigneeMicromass UK Limited
InventorsEmrys Jones, Steven Derek Pringle, Keith Richardson, James Ian Langridge, Zoltan Takats
Life of this patent
Estimating the term…
Abstract
A method of analysis using mass spectrometry and/or ion mobility spectrometry is disclosed comprising: (a) using a first device to generate smoke, aerosol or vapour from a target in vitro or ex vivo cell population; (b) mass analysing and/or ion mobility analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data; and (c) analysing said spectrometric data in order to identify and/or characterise said target cell population or one or more cells and/or compounds present in said target cell population.
Claims17
A method of analysis using mass spectrometry and/or ion mobility spectrometry comprising:
- using a first device to generate smoke, aerosol or vapour from a target in vitro cell line and/or culture medium derived therefrom;
- adding a matrix to said aerosol, smoke or vapour to dissolve at least some of the analytes within the aerosol, smoke or vapour, wherein said matrix comprises isopropanol;
- causing the dissolved aerosol, smoke or vapour, or analyte therein, to impact upon a collision surface located within a, or the, vacuum chamber of a mass spectrometer and/or ion mobility spectrometer so as to generate a plurality of analyte ions, wherein the matrix is added prior to the aerosol, smoke or vapour or analyte therein being impacted on a collision surface;
- mass analysing and/or ion mobility analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data;
- and
- analysing said spectrometric data in order to identify and/or characterise said target cell line or one or more cells and/or compounds present in said target cell line and/or culture medium derived therefrom;
- wherein said cell line comprises or consists of mutant and/or transgenic cells and wherein the cell line is a human or non-human animal cell line.
4 more elements
An apparatus comprising:
- a first device for generating smoke, aerosol or vapour from a target in vitro cell line and/or culture medium derived therefrom;
- a second device configured to add a matrix to said aerosol, smoke or vapour to dissolve at least some of the analytes within the aerosol, smoke or vapour;
- a collision surface located within a, or the, vacuum chamber of a mass spectrometer and/or ion mobility spectrometer, wherein the apparatus is configured to cause the dissolved aerosol, smoke or vapour, or analyte therein, to impact upon the collision surface so as to generate a plurality of analyte ions, wherein the apparatus is configured such that the matrix is added prior to the aerosol, smoke or vapour or analyte therein being impacted on the collision surface;
- a mass spectrometer and/or ion mobility spectrometer for analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data;
- and
- a processor adapted to analyse said spectrometric data in order to identify and/or characterise said target cell line or one or more cells and/or compounds present in said target cell line and/or culture medium derived therefrom;
- wherein said cell line comprises or consists of mutant and/or transgenic cells and wherein the cell line is a human or non-human animal cell line;
- and
- wherein said matrix comprises isopropanol;
- and/or
- wherein said apparatus comprises a sample transfer tube and a matrix introduction conduit, wherein said apparatus is configured to transfer the aerosol, smoke or vapour into a vacuum chamber of the mass spectrometer and/or ion mobility spectrometer via the sample transfer tube;
- wherein the apparatus is configured to add the matrix to said aerosol, smoke or vapour via the matrix introduction conduit;
- wherein the matrix introduction conduit has an inlet for receiving the matrix and an outlet that intersects with the sample transfer tube so as to allow the matrix to be intermixed with the smoke, aerosol or vapour in the sample transfer tube.
10 more elements
A method of analysis using mass spectrometry and/or ion mobility spectrometry comprising:
- using a first device to generate smoke, aerosol or vapour from a target in vitro cell line and/or culture medium derived therefrom;
- adding a matrix to said aerosol, smoke or vapour to dissolve at least some of the analytes within the aerosol, smoke or vapour;
- causing the dissolved aerosol, smoke or vapour, or analyte therein, to impact upon a collision surface located within a, or the, vacuum chamber of a mass spectrometer and/or ion mobility spectrometer so as to generate a plurality of analyte ions, wherein the matrix is added prior to the aerosol, smoke or vapour or analyte therein being impacted on a collision surface;
- mass analysing and/or ion mobility analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data;
- and
- analysing said spectrometric data in order to identify and/or characterise said target cell line or one or more cells and/or compounds present in said target cell line and/or culture medium derived therefrom;
- wherein said cell line comprises or consists of mutant and/or transgenic cells and wherein the cell line is a human or non-human animal cell line;
- and
- wherein said method comprises transferring the aerosol, smoke or vapour into a vacuum chamber of a mass spectrometer and/or ion mobility spectrometer via a sample transfer tube;
- and adding the matrix to said aerosol, smoke or vapour via a matrix introduction conduit;
- wherein the matrix introduction conduit has an inlet for receiving the matrix and an outlet that intersects with the sample transfer tube so as to allow the matrix to be intermixed with the smoke, aerosol or vapour in the sample transfer tube.
8 more elements
Dependencies are inferred from the claim text.