Optical arrangement and method for imaging a sample
AssigneeEuropean Molecular Biology Laboratory
InventorsGustavo Quintas Glasner de Medeiros, Lars Hufnagel, Nils Norlin
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Abstract
An optical arrangement for imaging a sample is disclosed. The optical arrangement comprises at least one first objective lens and at least one second objective lens, at least one illumination source for producing an illumination beam, detector for imaging radiation from the sample, and at least one mirror for reflecting the radiation from one of the first objective lens or the second objective lens into the detector. The at least one mirror is double-sided and dependent on the illumination beam at the other one of the first objective lens and the second objective lens.
Claims9
An optical arrangement for imaging a sample, comprising:
- an illumination source;
- a detector;
- a movable radiation selector;
- a plurality of light paths, ones of the plurality of light paths comprising
- an objective lens;
- an illumination light path for passing an illumination beam from the illumination source through the objective lens to illuminate the sample;
- a detection light path for passing radiation collected from the sample through the objective lens to the detector to image the sample;
- wherein the detection light paths meet at the movable radiation selector, and the movable radiation selector is configured to direct, in a predefined position of the movable radiation detector, the detection light path of a selected one of the plurality of light paths towards the detector.
A method of imaging a sample by means of a plurality of light paths, the method comprising
- selecting at least one illuminating one of the plurality of light paths for illuminating the sample
- selecting at least one detecting one of the plurality of light paths for detecting radiation from the sample, wherein the selecting of the at least one detecting one of the plurality of light paths comprises positioning in a predefined position a movable radiation selector,
- passing, by means of the at least one illuminating one of the plurality of light paths, an illumination beam from a light source via an illuminating objective lens to the sample,
- passing, by means of the at least one detecting one of the plurality of light paths, radiation from the sample via a detecting objective lens and via the movable radiation selector to the detector for detecting the radiation,
- processing the radiation to create first image data
- wherein the movable radiation selector is configured to direct the detecting one of the plurality of light paths towards the detector.
Dependencies are inferred from the claim text.