Device for monitoring the spatial and temporal dynamics of thrombin

A device for monitoring spatial and temporal dynamics of thrombin that includes:

• a temperature-controlled sealed chamber with a transparent window and a light trap, said temperature-controlled sealed chamber being filled with a fluid medium and designed to be capable of accommodating a cuvette containing a test sample of blood plasma, and a coagulation activating insert placed into the cuvette, the coagulation activating insert contains a butt end, wherein a substance applied to the butt end initiates a coagulation process, at least one means of illumination of the sample designed to be capable of exciting a light scattering signal from the sample, at least one first means of irradiation and at least one second means of irradiation, a means of optical photo/video registration of light scattering/irradiation from the sample, and a means for adjustment of pressure in the temperature-controlled sealed chamber designed to be capable of maintaining the pressure inside the temperature-controlled sealed chamber exceeding atmospheric pressure, wherein the at least one first means of irradiation is designed to be capable of exciting a fluorescence signal of a special marker that forms in the sample during cleavage of a fluorogenic substrate previously added to the sample by one of proteolytic enzymes of the coagulation system and the at least one second means of irradiation of the sample is designed to be capable of exciting the fluorescence signal of the said marker, where the at least one first means of irradiation provides irradiation of the sample through the transparent window in a direction perpendicular to a cuvette plane, and the at least one second means of irradiation provides irradiation of the sample through the transparent window at an angle to the cuvette plane from below the cuvette, wherein the butt end of the coagulation activating insert and a growing fibrin clot are irradiated,
• and wherein the at least one first means of irradiation and the at least one second means of irradiation have the same spectral range of irradiation that differs from the spectral range of the at least one means of illumination, and wherein the first and the second means of irradiation are switched on at the same time.